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Titel:

Biomass estimation in Pichia pastoris cultures by combined single-wavelength fluorescence measurements

Dokumenttyp:
Zeitschriftenaufsatz
Autor(en):
Brunner, Vincent; Hussein, Mohamed; Becker, Thomas
Abstract:
In this work, the evolution of different biogenic fluorophores involved in the metabolism of Pichia pastoris was determined at four different single-wavelength pairs (excitation/emission) during batch culture in microwell plates and used for effective and reliable biomass estimation by means of chemometric tools. The chemometric tools for biomass estimation were multiple linear regression (MLR), partial least squares regression (PLSR), and principal component regression (PCR). Variable importanc...     »
Stichworte:
Pichia pastoris bioprocess monitoring biomass estimation fluorescence spectroscopy partial least squares regression principal component regression
Zeitschriftentitel:
Biotechnology and Bioengineering
Jahr:
2016
Band / Volume:
113
Heft / Issue:
11
Seitenangaben Beitrag:
2394-2402
Volltext / DOI:
doi:10.1002/bit.26003
WWW:
http://dx.doi.org/10.1002/bit.26003
Print-ISSN:
1097-0290
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