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Titel:

Development of an epitope-specific analytical tool for the major peanut allergen Ara h 2 using a high-density multiple-antigenic peptide strategy

Dokumenttyp:
Zeitschriftenaufsatz
Autor(en):
Gruber, Patrick; Suhr, Martin; Frey, Andreas; Becker, Wolf-Meinhard; Hofmann, Thomas
Abstract:
Using the major peanut allergen Ara h 2 as an example, an analytical tool enabling the determination of immunoglobulin E (IgE)-epitopes in processed food allergens was developed. We synthesized a multiple-antigenic peptide (MAP) of the IgE-reactive linear epitope 3 (amino acid positions 27-36) of Ara h 2 and raised a monospecific antiserum against this epitope to obtain a positive control for future epitope resolved diagnostics. First, a MAP of epitope 3, having a molecular mass of 7770 Da, was...     »
Zeitschriftentitel:
Molecular nutrition & Food research
Jahr:
2004
Band / Volume:
48
Heft / Issue:
6
Seitenangaben Beitrag:
449--458
Volltext / DOI:
doi:10.1002/mnfr.200400005
Print-ISSN:
1613-4125
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