Detection of molecular biological objects, cellular biological objects and cell aggregates using quantitative phase-contrast microscopy

Disclosed herein is a method for detecting cell aggregates of biological cells using a quantitative phase-contrast microscope, a device for detecting cell aggregates of biological cells, a method for detecting cellular and/or molecular biological objects using a quantitative phase-contrast microscope and a device for detecting cellular and/or molecular biological objects. The method for detecting cell aggregates of biological cells using a quantitative phase-contrast microscope comprises preparing a suspension, the suspension comprising a viscoelastic fluid and biological cells from a sample, wherein the viscoelastic fluid comprises a shear-thinning polymer having a molecular weight between 2 MDa and 10 MDa and wherein a mass fraction of the shear-thinning polymer in the suspension is less than 0.2%. A flow of the suspension is generated along a microfluidic channel to viscoelastically focus cell aggregates in the suspension in a focal plane of the quantitative phase-contrast microscope. One or more phase shift images of the biological cells in the suspension are taken using the quantitative phase-contrast microscope and cell aggregates are identified in the one or more phase shift images.     «

Disclosed herein is a method for detecting cell aggregates of biological cells using a quantitative phase-contrast microscope, a device for detecting cell aggregates of biological cells, a method for detecting cellular and/or molecular biological objects using a quantitative phase-contrast microscope and a device for detecting cellular and/or molecular biological objects. The method for detecting cell aggregates of biological cells using a quantitative phase-contrast microscope comprises prepari...     »