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Title:

Trimodal Mixed Mode Chromatography That Enables Efficient Offline Two-Dimensional Peptide Fractionation for Proteome Analysis

Document type:
Zeitschriftenaufsatz
Author(s):
Yu, Peng; Petzoldt, Svenja; Wilhelm, Mathias; Zolg, Daniel Paul; Zheng, Runsheng; Sun, Xuefei; Liu, Xiaodong; Schneider, Günter; Huhmer, Andreas; Kuster, Bernhard
Abstract:
Offline two-dimensional chromatography is a common means to achieve deep proteome coverage. To reduce sample complexity and dynamic range and to utilize mass spectrometer (MS) time efficiently, high chromatographic resolution of and good orthogonality between the two dimensions are needed. Ion exchange and high pH reversed phase chromatography are often used for this purpose. However, the former requires desalting to be MS-compatible, and the latter requires fraction pooling to create orthogonal...     »
Journal title:
Analytical Chemistry
Year:
2017
Journal volume:
89
Journal issue:
17
Pages contribution:
8884-8891
Fulltext / DOI:
doi:10.1021/acs.analchem.7b01356
Publisher:
American Chemical Society (ACS)
E-ISSN:
0003-27001520-6882
Date of publication:
18.08.2017
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