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Title:

Improved In Vitro Model for Intranasal Mucosal Drug Delivery: Primary Olfactory and Respiratory Epithelial Cells Compared with the Permanent Nasal Cell Line RPMI 2650

Document type:
Article
Author(s):
Ladel, Simone; Schlossbauer, Patrick; Flamm, Johannes; Luksch, Harald; Mizaikoff, Boris; Schindowski, Katharina
Abstract:
Background: The epithelial layer of the nasal mucosa is the first barrier for drug permeation during intranasal drug delivery. With increasing interest for intranasal pathways, adequate in vitro models are required. Here, porcine olfactory (OEPC) and respiratory (REPC) primary cells were characterised against the nasal tumour cell line RPMI 2650. Methods: Culture conditions for primary cells from porcine nasal mucosa were optimized and the cells characterised via light microscope, RT-PCR and imm...     »
Keywords:
barrier model; nose-to-brain; primary cells; RPMI 2650; olfactory epithelium; respiratory epithelium
Journal title:
PHARMACEUTICS
Year:
2019
Journal volume:
11
Month:
AUG
Journal issue:
8
Language:
English
Fulltext / DOI:
doi:10.3390/pharmaceutics11080367
Publisher:
MDPI
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