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Titel:

Improved In Vitro Model for Intranasal Mucosal Drug Delivery: Primary Olfactory and Respiratory Epithelial Cells Compared with the Permanent Nasal Cell Line RPMI 2650

Dokumenttyp:
Article
Autor(en):
Ladel, Simone; Schlossbauer, Patrick; Flamm, Johannes; Luksch, Harald; Mizaikoff, Boris; Schindowski, Katharina
Abstract:
Background: The epithelial layer of the nasal mucosa is the first barrier for drug permeation during intranasal drug delivery. With increasing interest for intranasal pathways, adequate in vitro models are required. Here, porcine olfactory (OEPC) and respiratory (REPC) primary cells were characterised against the nasal tumour cell line RPMI 2650. Methods: Culture conditions for primary cells from porcine nasal mucosa were optimized and the cells characterised via light microscope, RT-PCR and imm...     »
Stichworte:
barrier model; nose-to-brain; primary cells; RPMI 2650; olfactory epithelium; respiratory epithelium
Zeitschriftentitel:
PHARMACEUTICS
Jahr:
2019
Band / Volume:
11
Monat:
AUG
Heft / Issue:
8
Sprache:
English
Volltext / DOI:
doi:10.3390/pharmaceutics11080367
Verlag / Institution:
MDPI
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