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Dokumenttyp:
Zeitschriftenaufsatz
Autor(en):
Heidebrecht, H.-J.; Kainz, B.; Schopf, R.; Godl, K.; Karcier, Z.; Kulozik, U.; Förster, B.
Titel:
Isolation of biofunctional bovine immunoglobulin G from milk- and colostral whey with mixed-mode chromatography at lab and pilot scale
Abstract:
The aim of the present work was to develop a new scalable and cost-efficient process to isolate bovine immunoglobulin G from colostral whey with high purity and minimal loss of activity. The mixed mode material Mercapto-Ethyl-Pyridine-HypercelTM was identified appropriate for direct capture of immunoglobulin G. The binding mechanism is primarily based on hydrophobic interactions at physiological conditions. As compared to immunoglobulin G, all other low molecular whey proteins such as ␣-Lactalbu...     »
Stichworte:
Hydrophobic charge-induction chromatography; Immunoglobulin G; Mixed-mode chromatography; Whey proteins
Zeitschriftentitel:
Journal of Chromatography A
Jahr:
2018
Band / Volume:
1562
Seitenangaben Beitrag:
59--68
Volltext / DOI:
doi:10.1016/j.chroma.2018.05.046
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