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Titel:

Lectin inhibition assays for the analysis of bioactive milk sialoglycoconjugates

Dokumenttyp:
Zeitschriftenaufsatz
Autor(en):
Koliwer-Brandl, H.; Siegert, N.; Umnus, K.; Kelm, A.; Tolkach, A.; Kulozik, U.; Kuballa, J.; Cartellieri, S.; Kelm, S.
Abstract:
Assay systems were developed to determine bioactive sialylated structures in complex and crude mixtures as they occur in milk fractionation processes. The sialic acid-binding proteins Maackia amurensis lectin and Sambucus nigra agglutinin as well as Siglec-2 and Siglec-4 were used in inhibition assays. The complementary linkage specificities of these lectins allow the determination of a2,3- and a2,6-linked sialic acids even in the presence of high lactose concentrations that are common in milk p...     »
Zeitschriftentitel:
International Dairy Journal
Jahr:
2011
Band / Volume:
21
Heft / Issue:
6
Seitenangaben Beitrag:
413--420
Volltext / DOI:
doi:10.1016/j.idairyj.2011.01.005
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