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Title:

Semi-quantitative, spatially resolved analysis of protein deposit layers on membrane surfaces

Document type:
Zeitschriftenaufsatz
Author(s):
Hartinger, Martin; Napiwotzki, Jonas; Schmid, Eva-Maria; Kurz, Franziska; Kulozik, Ulrich
Abstract:
Fouling distinctly reduces the filtration performance of membranes. A characterization of the fouling in membranes, however, is difficult due to its spatial distribution. Currently applied methods for deposit layer analysis are rather complex or do not offer a spatial resolution. Knowledge of the spatial distribution, however, could be used to improve the design of membranes, modules, and spacers. Staining with Coomassie Brilliant Blue, related to the staining of PAGE gels, is a simple method to v...     »
Keywords:
Coomassie Brilliant Blue; deposit layer; Polymeric membrane; Protein; Quantification; Semi-quantitative, spatially resolved analysis of protein deposit layers on membrane surfaces; skim milk; Spatially resolved analysis; Staining; SWM; Visualization
Journal title:
MethodsX
Year:
2020
Journal volume:
7
Pages contribution:
100780
Fulltext / DOI:
doi:10.1016/j.mex.2019.100780
Print-ISSN:
2215-0161
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