Reaction kinetic pathway of reversible and irreversible thermal denaturation of β-lactoglobulin

A reaction kinetic approach for the description of reversible (unfolding) and irreversible (aggregation) denaturation of -lactoglobulin (-Lg) was developed in this study. This novel approach allows the calculation of the content of native, partially unfolded and irreversibly denatured -Lg as a function of the heat treatment time. Moreover, it is able to give a mathematical interpretation of the sharp bend on the Arrhenius plot of the formal denaturation rate constant which is characteristic of the denaturation behaviour of both major whey proteins, -lactalbumin and -lactoglobulin. The developed theoretical backgrounds were applied for the denaturation kinetics of -Lg in a protein solution containing 50 gL-1 of the isolated whey protein fraction and verified by means of differential scanning calorimetry.     «

A reaction kinetic approach for the description of reversible (unfolding) and irreversible (aggregation) denaturation of -lactoglobulin (-Lg) was developed in this study. This novel approach allows the calculation of the content of native, partially unfolded and irreversibly denatured -Lg as a function of the heat treatment time. Moreover, it is able to give a mathematical interpretation of the sharp bend on the Arrhenius plot of the formal denaturation rate constant which is characteristic of t...     »