Benutzer: Gast  Login
Titel:

Quantitative assessment of thermal denaturation of bovine α-lactalbumin via low-intensity ultrasound, HPLC, and DSC

Dokumenttyp:
Zeitschriftenaufsatz
Autor(en):
Wang, Q.; Tolkach, A.; Kulozik, U.
Abstract:
The degree of irreversible aggregation and the aggregation velocity constant of alpha-lactalbumin (alpha-la) were determined by three methods based on different principles: low-intensity ultrasound as a novel method for this purpose, DSC, and HPLC. The denaturation process of alpha-la causes a decrease in the ultrasonic velocity due to the conformation change of alpha-la molecules. This decrease is a function of the concentration of native alpha-la in the sample. A linear correlation was found b...     »
Stichworte:
Animals; Calorimetry, Differential Scanning; Cattle; Chromatography, High Pressure Liquid; Hot Temperature; Kinetics; Lactalbumin/chemistry; Protein denaturation; Ultrasonics
Zeitschriftentitel:
Journal of Agricultural and Food Chemistry
Jahr:
2006
Band / Volume:
54
Heft / Issue:
18
Seitenangaben Beitrag:
6501--6506
Volltext / DOI:
doi:10.1021/jf060895k
 BibTeX