This thesis demonstrates the potential of MALDI-TOF MS to differentiate beverage associated yeasts at a previously not achievable level. Consequently, yeasts can be assessed for their spoilage potential or predicted for their possible application in specific brewing and wine making processes, which has not been possible with any other previously used (molecular) techniques with so little effort. This achievement is based on the optimization of sample preparation, evaluation of the impact of culturing conditions, enlargement of the proteome database, and development of new bioinformatics algorithms for data analyses and biomarker definition.
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This thesis demonstrates the potential of MALDI-TOF MS to differentiate beverage associated yeasts at a previously not achievable level. Consequently, yeasts can be assessed for their spoilage potential or predicted for their possible application in specific brewing and wine making processes, which has not been possible with any other previously used (molecular) techniques with so little effort. This achievement is based on the optimization of sample preparation, evaluation of the impact of cult...
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