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- Title:
Hydrolysis of β-lactoglobulin by trypsin under acidic pH and analysis of the hydrolysates with MALDI–TOF–MS/MS
- Document type:
- Zeitschriftenaufsatz
- Author(s):
- Cheison, S. C.; Lai, M.-Y.; Leeb, E.; Kulozik, U.
- Abstract:
- Trypsin (EC 3.4.21.4) hydrolysis of food proteins are done at the optimum pH (7.8) and temperature (37 C). Little information is available on the effect of sub-optimal conditions on hydrolysis. Bovine b-lactoglobulin (b-Lg) was hydrolysed by trypsin under acidic pH (pH 4–7) between 20 and 60 C and the substrate concentration from 2.5% to 15% (w/v) and compared with hydrolysis at pH 7.8 and 37 C. Aliquots were taken at different times (t = 0 up to 10 min). Samples were analysed using matrix-assisted laser desorption/ionisation time-of-flight tandem mass spectrometry (MALDI–TOF–MS/MS) with a-cyano-4hydroxycinnamic acid (HCCA) and 2,5-dihydroxyacetophenone (DHAP) matrices. Hydrolysis patterns of b-Lg were generally similar at pH 7.8, 7, 6 and 5 while at pH 4 fewer peptides were detected except a unique fragment f(136–141). The different cleavage sites of b-Lg showed low resistance to trypsin at optimum conditions and pH 7 while being random and simultaneous. At lower pH, some cleavage sites showed increased resistance, while hydrolysis was relatively slow and ordered. Initial attack by trypsin occurred at Arg40–Val41, Lys141–Ala142 and Arg148–Leu149 resistance was at Lys60–Trp61, Arg124–Thr125 and Lys135–Phe136. Five domains were identified based on b-Lg resistance to trypsin in the order f(1– 40) < f(41–75) < f(76–91) > f(92–138) > f(139–162). Results suggest that hydrolysis away from trypsin optimum offer better hydrolysis process control and different peptides. This strategy may be used to protect target bioactive or precursor peptides, or avoid the production of unwanted peptides.
- Keywords:
- Acid pH; Hydrolysis resistance; Matrix assisted laser desorption/ionisation; Time of flight tandem mass spectrometry; Trypsin optimum conditions
- Journal title:
- Food Chemistry
- Year:
- 2011
- Journal volume:
- 125
- Pages contribution:
- 1241--1248
- Fulltext / DOI:
- doi:10.1016/j.foodchem.2010.10.042
BibTeX
Occurrences:
- mediaTUM GesamtbestandHochschulbibliographie2011Zentrale EinrichtungenZIEL - Institute for Food & Health
- mediaTUM GesamtbestandHochschulbibliographie2011FakultätenWissenschaftszentrum WeihenstephanLehrstuhl für Lebensmittelverfahrenstechnik und Molkereitechnik (Prof. Kulozik)