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Titel:

Recordings from human myenteric neurons using voltage-sensitive dyes.

Dokumenttyp:
Zeitschriftenaufsatz
Autor(en):
Vignali, Sheila; Peter, Nadine; Ceyhan, Guralp; Demir, Ihsan Ekin; Zeller, Florian; Senseman, David; Michel, Klaus; Schemann, Michael
Abstract:
Voltage-sensitive dye (VSD) imaging became a powerful tool to detect neural activity in the enteric nervous system, including its routine use in submucous neurons in freshly dissected human tissue. However, VSD imaging of human myenteric neurons remained a challenge because of limited visibility of the ganglia and dye accessibility. We describe a protocol to apply VSD for recordings of human myenteric neurons in freshly dissected tissue and myenteric neurons in primary cultures. VSD imaging of g...     »
Stichworte:
Cells, Cultured, Signal Processing, Computer-Assisted, Immunohistochemistry, Electric Stimulation, Animals, Humans, Guinea Pigs, Male, Pyridinium Compounds/pharmacology, Excitatory Postsynaptic Potentials/drug effects/physiology, Action Potentials/drug effects/physiology, Electrophysiology/*methods, Fluorescent Dyes/*pharmacology, Microscopy, Fluorescence/methods, Myenteric Plexus/*cytology, Neurons/*cytology/drug effects/physiology
Zeitschriftentitel:
Journal of neuroscience methods
Jahr:
2010
Band / Volume:
192
Monat:
oct
Heft / Issue:
2
Seitenangaben Beitrag:
240--248
Sprache:
eng
Volltext / DOI:
doi:10.1016/j.jneumeth.2010.07.038
Print-ISSN:
1872-678X 0165-0270
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