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Title:

Enzyme expression kinetics by Escherichia coli during transition from rich to minimal media depends on proteome reserves

Document type:
Zeitschriftenaufsatz
Author(s):
Wu, Chenhao; Mori, Matteo; Abele, Miriam; Banaei-Esfahani, Amir; Zhang, Zhongge; Okano, Hiroyuki; Aebersold, Ruedi; Ludwig, Christina; Hwa, Terence
Abstract:
Bacterial fitness depends on adaptability to changing environments. In rich growth medium, which is replete with amino acids, Escherichia coli primarily expresses protein synthesis machineries, which comprise ~40% of cellular proteins and are required for rapid growth. Upon transition to minimal medium, which lacks amino acids, biosynthetic enzymes are synthesized, eventually reaching ~15% of cellular proteins when growth fully resumes. We applied quantitative proteomics to analyse the timing of...     »
Keywords:
BayBioMS; Microbiology, Systems analysis
Journal title:
Nature Microbiology
Year:
2023
Journal volume:
8
Journal issue:
2
Pages contribution:
347-359
Fulltext / DOI:
doi:10.1038/s41564-022-01310-w
Publisher:
Springer Science and Business Media LLC
E-ISSN:
2058-5276
Date of publication:
03.02.2023
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