Linke, K., Periasamy, N., Ehrmann, M., Winter, R. and Vogel, R. F.
Influence of high pressure on the dimerization of ToxR, a protein involved in bacterial signal transduction.
High hydrostatic pressure (HHP) is suggested to influence the structure and function of membranes and/or integrated proteins. We demonstrate for the first time HHP-induced dimer dissociation of membrane proteins in vivo with Vibrio cholerae ToxR variants in Escherichia coli reporter strains carrying ctx:lacZ fusions. Dimerization ceased at 20 to 50 MPa depending on the nature of the transmembrane segments rather than on changes in the ToxR lipid bilayer environment.