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Title:

Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission.

Document type:
Journal Article
Author(s):
Aggarwal, Abhi; Liu, Rui; Chen, Yang; Ralowicz, Amelia J; Bergerson, Samuel J; Tomaska, Filip; Mohar, Boaz; Hanson, Timothy L; Hasseman, Jeremy P; Reep, Daniel; Tsegaye, Getahun; Yao, Pantong; Ji, Xiang; Kloos, Marinus; Walpita, Deepika; Patel, Ronak; Mohr, Manuel A; Tillberg, Paul W; Looger, Loren L; Marvin, Jonathan S; Hoppa, Michael B; Konnerth, Arthur; Kleinfeld, David; Schreiter, Eric R; Podgorski, Kaspar
Abstract:
The fluorescent glutamate indicator iGluSnFR enables imaging of neurotransmission with genetic and molecular specificity. However, existing iGluSnFR variants exhibit low in vivo signal-to-noise ratios, saturating activation kinetics and exclusion from postsynaptic densities. Using a multiassay screen in bacteria, soluble protein and cultured neurons, we generated variants with improved signal-to-noise ratios and kinetics. We developed surface display constructs that improve iGluSnFR's nanoscopic...     »
Journal title abbreviation:
Nat Methods
Year:
2023
Journal volume:
20
Journal issue:
6
Pages contribution:
925-934
Fulltext / DOI:
doi:10.1038/s41592-023-01863-6
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/37142767
Print-ISSN:
1548-7091
TUM Institution:
Institut für Neurowissenschaften (Prof. Konnerth)
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