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Title:

Linking post-translational modifications and protein turnover by site-resolved protein turnover profiling

Document type:
Zeitschriftenaufsatz
Author(s):
Zecha, Jana; Gabriel, Wassim; Spallek, Ria; Chang, Yun-Chien; Mergner, Julia; Wilhelm, Mathias; Bassermann, Florian; Kuster, Bernhard
Abstract:
Proteome-wide measurements of protein turnover have largely ignored the impact of post-translational modifications (PTMs). To address this gap, we employ stable isotope labeling and mass spectrometry to measure the turnover of >120,000 peptidoforms including >33,000 phosphorylated, acetylated, and ubiquitinated peptides for >9,000 native proteins. This site-resolved protein turnover (SPOT) profiling discloses global and site-specific differences in turnover associated with the presence or absenc...     »
Keywords:
BayBioMS; Mass spectrometry; Post-translational modifications; Proteolysis; Proteomics
Journal title:
Nature Communications
Year:
2022
Journal volume:
13
Journal issue:
1
Fulltext / DOI:
doi:10.1038/s41467-021-27639-0
Publisher:
Springer Science and Business Media LLC
E-ISSN:
2041-1723
Date of publication:
10.01.2022
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