The effect of transglutaminase and xylanase on arabinoxylans and proteins in rye doughs was examined by RP- and GP-HPLC. Transglutaminase caused a decrease of the prolamin and an increase of the glutelin fraction. This improved the aggregation of rye proteins, which was not further affected by xylanase. Fractionation-reconstitution studies were conducted to determine the functional properties of rye flour constituents. For this purpose, a method for the isolation of a rye protein fraction with high protein content was developed for the first time.
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The effect of transglutaminase and xylanase on arabinoxylans and proteins in rye doughs was examined by RP- and GP-HPLC. Transglutaminase caused a decrease of the prolamin and an increase of the glutelin fraction. This improved the aggregation of rye proteins, which was not further affected by xylanase. Fractionation-reconstitution studies were conducted to determine the functional properties of rye flour constituents. For this purpose, a method for the isolation of a rye protein fraction with h...
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