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Document type:
Journal Article; Research Support, Non-U.S. Gov't; Article
Author(s):
Adelsberger, Helmuth; Grienberger, Christine; Stroh, Albrecht; Konnerth, Arthur
Title:
In vivo calcium recordings and channelrhodopsin-2 activation through an optical fiber.
Abstract:
We describe here an approach for the fluorometric monitoring of population activity in neurons in live mice combined with the activation of optogenetic actuators in vivo. In this protocol, a thin multimode fiber, which is used for both delivering excitation light and collecting emitted fluorescence signals, is inserted into the skull of a mouse. When combined with multicell bolus loading of Ca(2+) indicators, this optical fiber and its associated fluorescence detection system can be used for the...    »
Journal title abbreviation:
Cold Spring Harb Protoc
Year:
2014
Journal volume:
2014
Journal issue:
10
Pages contribution:
pdb.prot084145
Language:
eng
Print-ISSN:
1940-3402
TUM Institution:
Institut für Neurowissenschaften
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