Journal Article; Research Support, Non-U.S. Gov't; Article
Heim, N; Garaschuk, O; Friedrich, MW; Mank, M; Milos, RI; Kovalchuk, Y; Konnerth, A; Griesbeck, O
Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor.
Fluorescent Ca(2+) indicator proteins (FCIPs) are attractive tools for studying Ca(2+) dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca(2+) sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca(2+) imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits.