Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor.

Titel:: Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor.
Dokumenttyp:: Journal Article; Research Support, Non-U.S. Gov't; Article
Autor(en):: Heim, N; Garaschuk, O; Friedrich, MW; Mank, M; Milos, RI; Kovalchuk, Y; Konnerth, A; Griesbeck, O
Abstract:: Fluorescent Ca(2+) indicator proteins (FCIPs) are attractive tools for studying Ca(2+) dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca(2+) sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca(2+) imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits.
Zeitschriftentitel:: Nat Methods
Jahr:: 2007
Band / Volume:: 4
Heft / Issue:: 2
Seitenangaben Beitrag:: 127-9
Sprache:: eng
Volltext / DOI:: doi:10.1038/nmeth1009
PubMed:: http://view.ncbi.nlm.nih.gov/pubmed/17259991
Print-ISSN:: 1548-7091
TUM Einrichtung:: Institut für Neurowissenschaften
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