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Titel:

Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor.

Dokumenttyp:
Journal Article; Research Support, Non-U.S. Gov't; Article
Autor(en):
Heim, N; Garaschuk, O; Friedrich, MW; Mank, M; Milos, RI; Kovalchuk, Y; Konnerth, A; Griesbeck, O
Abstract:
Fluorescent Ca(2+) indicator proteins (FCIPs) are attractive tools for studying Ca(2+) dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca(2+) sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca(2+) imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits.
Zeitschriftentitel:
Nat Methods
Jahr:
2007
Band / Volume:
4
Heft / Issue:
2
Seitenangaben Beitrag:
127-9
Sprache:
eng
Volltext / DOI:
doi:10.1038/nmeth1009
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/17259991
Print-ISSN:
1548-7091
TUM Einrichtung:
Institut für Neurowissenschaften
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