In labs, microbes are often grown alone for simplicity. Yet, in nature, they thrive in complex communities. This thesis investigates a synthetic co-culture of Synechococcus elongatus PCC 7942 cscB feeding Pseudomonas putida derivatives with sucrose. The results present a highly controllable co-cultivation process that doubled the polyhydroxyalkanoate (PHA) titer compared to previous studies. Further, the co-partners showed multi-level interactions, including transcriptional, proteomic, and metabolic changes, as well as highly increased cyanomaberium growth.
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In labs, microbes are often grown alone for simplicity. Yet, in nature, they thrive in complex communities. This thesis investigates a synthetic co-culture of Synechococcus elongatus PCC 7942 cscB feeding Pseudomonas putida derivatives with sucrose. The results present a highly controllable co-cultivation process that doubled the polyhydroxyalkanoate (PHA) titer compared to previous studies. Further, the co-partners showed multi-level interactions, including transcriptional, proteomic, and metab...
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