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- Original title:
- Studying in vitro-differentiated hepatocytes by proteomics
- Translated title:
- Untersuchung von in vitro-differenzierten Hepatozyten mittels Proteomik
- Author:
- Krumm, Johannes
- Year:
- 2022
- Document type:
- Dissertation
- Faculty/School:
- TUM School of Life Sciences
- Advisor:
- Küster, Bernhard (Prof. Dr.)
- Referee:
- Küster, Bernhard (Prof. Dr.); Reichert, Maximilian (Prof. Dr.)
- Language:
- en
- Subject group:
- BIO Biowissenschaften
- Keywords:
- hepatocytes, cell differentiation, proteomics, mass spectrometry
- Translated keywords:
- Hepatozyten, Zelldifferenzierung, Proteomik, Massenspektrometrie
- TUM classification:
- CHE 828
- Abstract:
- Hepatocytes are the main parenchymal cell type of the liver and are, for example, widely used for assessing toxicity of bioactive compounds. Here, high-temporal proteome, phosphoproteome, and acetylome data of in vitro-derived hepatocytes were acquired using mass spectrometry. The analysis revealed multiple novel marker proteins and a major molecular switch impacting, e.g. metabolism, cell cycle, and epigenetic modifier. Further, comparing 2D and 3D hepatocyte differentiations against fetal and adult liver samples indicated a more fetal-like status of the in vitro models.
- Translated abstract:
- Hepatozyten sind der wichtigste parenchymale Zelltyp der Leber und werden beispielsweise für die Bewertung der Toxizität von bioaktiven Substanzen verwendet. Hier wurden mittels Massenspektrometrie hochtemporale Proteome-, Phosphoproteome- und Acetylom-Daten von in-vitro hergestellten Hepatozyten erfasst. Die Analyse ergab einige neue Markerproteine und einen großen molekularen Wechsel welcher z.B. den Metabolismus, den Zellzyklus und epigentische Modifikatoren betrifft. Außerdem ergab der Vergl... »
- WWW:
- https://mediatum.ub.tum.de/?id=1638992
- Date of submission:
- 01.02.2022
- Oral examination:
- 29.07.2022
- File size:
- 6167559 bytes
- Pages:
- 153
- Urn (citeable URL):
- https://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:91-diss-20220729-1638992-1-8
- Last change:
- 14.10.2022
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