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Title:

Targeting genomic SARS-CoV-2 RNA with siRNAs allows efficient inhibition of viral replication and spread.

Document type:
Article; Journal Article; Research Support, Non-U.S. Gov't
Author(s):
Ambike, Shubhankar; Cheng, Cho-Chin; Feuerherd, Martin; Velkov, Stoyan; Baldassi, Domizia; Afridi, Suliman Qadir; Porras-Gonzalez, Diana; Wei, Xin; Hagen, Philipp; Kneidinger, Nikolaus; Stoleriu, Mircea Gabriel; Grass, Vincent; Burgstaller, Gerald; Pichlmair, Andreas; Merkel, Olivia M; Ko, Chunkyu; Michler, Thomas
Abstract:
A promising approach to tackle the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) could be small interfering (si)RNAs. So far it is unclear, which viral replication steps can be efficiently inhibited with siRNAs. Here, we report that siRNAs can target genomic RNA (gRNA) of SARS-CoV-2 after cell entry, and thereby terminate replication before start of transcription and prevent virus-induced cell death. Coronaviruses replicate via negative sense RNA intermediates using a unique disco...     »
Journal title abbreviation:
Nucleic Acids Res
Year:
2022
Journal volume:
50
Journal issue:
1
Pages contribution:
333-349
Fulltext / DOI:
doi:10.1093/nar/gkab1248
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/34928377
Print-ISSN:
0305-1048
TUM Institution:
604; 663; Institut für Virologie (Prof. Protzer)
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