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Title:

Quantitative single-cell RT-PCR and Ca(2+) imaging in brain slices.

Document type:
Journal Article; Article
Author(s):
Durand, GM; Marandi, N; Herberger, SD; Blum, R; Konnerth, A
Abstract:
We have established a quantitative reverse transcriptase-PCR (RT-PCR) approach for the analysis of RNA transcript levels in individual cells of living brain slices. Quantification is achieved by using rapid-cycle, real-time PCR protocols and high-resolution external cDNA standard curves for the gene of interest. The method consists of several procedures, including cell soma harvest, reverse transcription, and an optimized cDNA purification step, which allowed us to quantify transcripts in small...     »
Journal title abbreviation:
Pflugers Arch
Year:
2006
Journal volume:
451
Journal issue:
6
Pages contribution:
716-726
Language:
eng
Fulltext / DOI:
doi:10.1007/s00424-005-1514-3
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/16211366
Print-ISSN:
0031-6768
TUM Institution:
Institut für Neurowissenschaften
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