Benutzer: Gast  Login
Titel:

In vivo calcium recordings and channelrhodopsin-2 activation through an optical fiber.

Dokumenttyp:
Journal Article; Research Support, Non-U.S. Gov't; Article
Autor(en):
Adelsberger, Helmuth; Grienberger, Christine; Stroh, Albrecht; Konnerth, Arthur
Abstract:
We describe here an approach for the fluorometric monitoring of population activity in neurons in live mice combined with the activation of optogenetic actuators in vivo. In this protocol, a thin multimode fiber, which is used for both delivering excitation light and collecting emitted fluorescence signals, is inserted into the skull of a mouse. When combined with multicell bolus loading of Ca(2+) indicators, this optical fiber and its associated fluorescence detection system can be used for the...     »
Zeitschriftentitel:
Cold Spring Harb Protoc
Jahr:
2014
Band / Volume:
2014
Heft / Issue:
10
Seitenangaben Beitrag:
pdb.prot084145
Sprache:
eng
Volltext / DOI:
doi:10.1101/pdb.prot084145
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/25275110
Print-ISSN:
1940-3402
TUM Einrichtung:
Institut für Neurowissenschaften
 BibTeX