Hydrolytic bacteria supply intermediates at the conversion of biomass to methane. In this work cellulolytic bacteria and their application as inoculum for the optimization of this process were investigated. In enrichments from environmental samples, Clostridium (C.) thermocellum, C. stercorarium and C. straminisolvens were found as cellulolytic species, which belong to the clostridia cluster III, and additionally C. cellulosi. In contrast, in biogas plants there were many uncultured species from cluster III identified. New C. thermocellum strains isolated from the samples showed physiological and genetical differences (cipA gene). The methane yield in a pilot plant was enhanced by addition of a particularly efficient hydrolytic strain in comparison to the autochthonous bacterial community.
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Hydrolytic bacteria supply intermediates at the conversion of biomass to methane. In this work cellulolytic bacteria and their application as inoculum for the optimization of this process were investigated. In enrichments from environmental samples, Clostridium (C.) thermocellum, C. stercorarium and C. straminisolvens were found as cellulolytic species, which belong to the clostridia cluster III, and additionally C. cellulosi. In contrast, in biogas plants there were many uncultured species from...
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