Treatment of wheat flour by ball and centrifugal milling induced protein modifications by thiol-disulfide interchange reactions. Intense treatments impaired the techno-functional properties, whereas weakly treated flours were suitable functional additives for base flours that led to an improvement of the baking quality. Also, an LC-MS/MS method to quantitate puroindolins (PIN), which are related to the hardness of the endosperm of wheat grains, was developed. Wheat cultivars with wild-type PINs showed higher PIN contents than cultivars with PIN sequence variations that had a hard endosperm texture and good baking performance.
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Treatment of wheat flour by ball and centrifugal milling induced protein modifications by thiol-disulfide interchange reactions. Intense treatments impaired the techno-functional properties, whereas weakly treated flours were suitable functional additives for base flours that led to an improvement of the baking quality. Also, an LC-MS/MS method to quantitate puroindolins (PIN), which are related to the hardness of the endosperm of wheat grains, was developed. Wheat cultivars with wild-type PINs...
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