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Titel:

Shack-Hartmann wave front measurements in cortical tissue for deconvolution of large three-dimensional mosaic transmitted light brightfield micrographs.

Dokumenttyp:
Evaluation Studies; Journal Article
Autor(en):
Oberlaender, M; Broser, PJ; Sakmann, B; Hippler, S
Abstract:
We present a novel approach for deconvolution of 3D image stacks of cortical tissue taken by mosaic/optical-sectioning technology, using a transmitted light brightfield microscope. Mosaic/optical-sectioning offers the possibility of imaging large volumes (e.g. from cortical sections) on a millimetre scale at sub-micrometre resolution. However, a blurred contribution from out-of-focus light results in an image quality that usually prohibits 3D quantitative analysis. Such quantitative analysis is...     »
Zeitschriftentitel:
J Microsc
Jahr:
2009
Band / Volume:
233
Heft / Issue:
2
Seitenangaben Beitrag:
275-89
Sprache:
eng
Volltext / DOI:
doi:10.1111/j.1365-2818.2009.03118.x
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/19220694
Print-ISSN:
0022-2720
TUM Einrichtung:
Institut für Neurowissenschaften
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