In the present thesis the development of a transcriptome map concerning malting quality for a spring barley population is described. In this map cDNA-AFLP marker, based on the differential gene expression during malting, were combined with genomic marker data of AFLPs, SSRs and SNPs. The constructed map was used for a QTL analysis with important malting quality parameters to estimate the exact contribution of the expressed transcripts to malting quality. QTL cluster mainly on chromosome 3H and 5H were associated with cDNA-AFLP markers. A sequence analysis indicated, that a part of the QTL flanking transcripts showed homologies with genes, coding for components of signal transduction pathways. Two transcriptome markers were converted into sequence-specific PCR-based markers, which are suitable for the selection concerning malting quality.
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In the present thesis the development of a transcriptome map concerning malting quality for a spring barley population is described. In this map cDNA-AFLP marker, based on the differential gene expression during malting, were combined with genomic marker data of AFLPs, SSRs and SNPs. The constructed map was used for a QTL analysis with important malting quality parameters to estimate the exact contribution of the expressed transcripts to malting quality. QTL cluster mainly on chromosome 3H and 5...
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