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Titel:

Transmitted light brightfield mosaic microscopy for three-dimensional tracing of single neuron morphology.

Dokumenttyp:
Comparative Study; Evaluation Studies; Journal Article
Autor(en):
Oberlaender, M; Bruno, RM; Sakmann, B; Broser, PJ
Abstract:
A fundamental challenge in neuroscience is the determination of the three-dimensional (3D) morphology of neurons in the cortex. Here we describe a semiautomated method to trace single biocytin-filled neurons using a transmitted light brightfield microscope. The method includes 3D tracing of dendritic trees and axonal arbors from image stacks of serial 100-microm-thick tangential brain sections. Key functionalities include mosaic scanning and optical sectioning, high-resolution image restoration,...     »
Zeitschriftentitel:
J Biomed Opt
Jahr:
2007
Band / Volume:
12
Heft / Issue:
6
Seitenangaben Beitrag:
064029
Sprache:
eng
Volltext / DOI:
doi:10.1117/1.2815693
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/18163845
Print-ISSN:
1083-3668
TUM Einrichtung:
Institut für Neurowissenschaften
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