RNA-mediated gene silencing signals are not graft transmissible from the rootstock to the scion in greenhouse-grown apple plants Malus sp

RNA silencing describes the sequence specific degradation of RNA targets. Silencing is a non-cell autonomous event that is graft transmissible in different plant species. The present study is the first report on systemic acquired dsRNA-mediated gene silencing of transgenic and endogenous gene sequences in a woody plant like apple. Transgenic apple plants overexpressing a hairpin gene construct of the gusA reporter gene were produced. These plants were used as rootstocks and grafted with scions of the gusA overexpressing transgenic apple clone T355. After grafting, we observed a reduction of the gusA gene expression in T355 scions in vitro, but not in T355 scions grown in the greenhouse. Similar results were obtained after silencing of the endogenous Mdans gene in apple that is responsible for anthocyanin biosynthesis. Subsequently, we performed grafting experiments with Mdans silenced rootstocks and red leaf scions of TNR31-35 in order to evaluate graft transmitted silencing of the endogenous Mdans. The results obtained suggested a graft transmission of silencing signals in in vitro shoots. In contrast, no graft transmission of dsRNA-mediated gene silencing signals was detectable in greenhouse-grown plants and in plants grown in an insect protection tent.     «

RNA silencing describes the sequence specific degradation of RNA targets. Silencing is a non-cell autonomous event that is graft transmissible in different plant species. The present study is the first report on systemic acquired dsRNA-mediated gene silencing of transgenic and endogenous gene sequences in a woody plant like apple. Transgenic apple plants overexpressing a hairpin gene construct of the gusA reporter gene were produced. These plants were used as rootstocks and grafted with scions o...     »

Agrobacterium tumefaciens/physiology; Disease Resistance/genetics; Gene Expression Regulation, Plant; Gene Silencing; Malus/genetics/immunology/microbiology; Plant Diseases/genetics/immunology/microbiology; Plant Proteins/genetics; Plant Roots/immunology/metabolism/microbiology; Plants, Genetically Modified/genetics/immunology/microbiology; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger/antagonists {&} inhibitors/genetics; RNA, Small Interfering/genetics     «

Agrobacterium tumefaciens/physiology; Disease Resistance/genetics; Gene Expression Regulation, Plant; Gene Silencing; Malus/genetics/immunology/microbiology; Plant Diseases/genetics/immunology/microbiology; Plant Proteins/genetics; Plant Roots/immunology/metabolism/microbiology; Plants, Genetically Modified/genetics/immunology/microbiology; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger/antagonists {&} inhibitors/genetics; RNA, Small Interfer...     »