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Title:

Silencing of flavanone-3-hydroxylase in apple (Malus $\times$ domestica Borkh.) leads to accumulation of flavanones, but not to reduced fire blight susceptibility

Document type:
Zeitschriftenaufsatz
Author(s):
Flachowsky, Henryk; Halbwirth, Heidi; Treutter, Dieter; Richter, Klaus; Hanke, Magda-Viola; Szankowski, Iris; Gosch, Christian; Stich, Karl; Fischer, Thilo C.
Abstract:
Transgenic antisense flavanone-3-hydroxylase apple plants were produced to mimic the effect of the agrochemical prohexadione-Ca on apple leaves. This enzyme inhibitor for 2-oxoglutarate dependent dioxygenases is used as a growth retardant and for control of secondary fire blight of leaves. Like using the agent, silencing of flavanone-3-hydroxylase leads to an accumulation of flavanones in leaves, but in contrast not to the formation of 3-deoxyflavonoids. In prohexadione-Ca treated leaves the 3-d...     »
Keywords:
Alcohol Oxidoreductases/metabolism; Anti-Bacterial Agents/metabolism/pharmacology; Benzopyrans/metabolism/pharmacology; Cloning, Molecular; Culture Media/metabolism; Disease Susceptibility/immunology/microbiology; Enzyme Activation; Enzyme Inhibitors/pharmacology; Erwinia amylovora/drug effects/immunology/pathogenicity; Flavanones/biosynthesis/genetics; Gene Expression Regulation, Plant; Gene Silencing; Genes, Plant; Genetic Vectors/genetics/metabolism; Ketoglutaric Acids/pharmacology; Malus/enz...     »
Journal title:
Plant physiology and biochemistry : PPB
Year:
2012
Journal volume:
51
Pages contribution:
18--25
Fulltext / DOI:
doi:10.1016/j.plaphy.2011.10.004
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