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Title:

Are histones the targets for flavan-3-ols (catechins) in nuclei?

Document type:
Zeitschriftenaufsatz
Author(s):
Polster, Jürgen; Dithmar, Heike; Walter, Feucht
Abstract:
Binding of flavan-3-ols to nuclei is characteristic of Tsuga canadensis (coniferous tree). This is achieved with the DMACA reagent (p-dimethylamino-cinnamaldehyde), which stains almost exclusively monomeric and oligomeric flavan-3-ols with an intense blue colour. Deep flavanol staining also occurred when calf cells of small intestine were enriched with added catechins. In order to detect the components of nuclei that may associate with catechins, the principal components of chromatin (DNA, histo...     »
Keywords:
Catechin/analogs {&} derivatives/analysis/chemistry/metabolism; Cell Nucleus/chemistry/ultrastructure; Cells, Cultured; Chromatin/chemistry/metabolism; Chromosomes/chemistry/metabolism/ultrastructure; DNA/chemistry/metabolism; Flavonoids/chemistry; Histocytochemistry; Histones/analysis/chemistry/metabolism; Hydrogen-Ion Concentration; Mathematics; Mitosis; Spectrum Analysis; Titrimetry; Tsuga/chemistry/metabolism
Journal title:
Biological chemistry
Year:
2003
Journal volume:
384
Journal issue:
7
Pages contribution:
997--1006
Fulltext / DOI:
doi:10.1515/BC.2003.112
Print-ISSN:
1431-6730
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