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Title:

Population imaging of synaptically released glutamate in mouse hippocampal slices.

Document type:
Journal Article; Research Support, Non-U.S. Gov't
Author(s):
Unger, Felix; Konnerth, Arthur; Zott, Benedikt
Abstract:
Glutamatergic neurotransmission is a widespread form of synaptic excitation in the mammalian brain. The development of genetically encoded fluorescent glutamate sensors allows monitoring synaptic signaling in living brain tissue in real time. Here, we describe single- and two-photon imaging of synaptically evoked glutamatergic population signals in acute hippocampal slices expressing the fluorescent glutamate sensor SF-iGluSnFR.A184S in CA1 or CA3 pyramidal neurons. The protocol can be readily u...     »
Journal title abbreviation:
STAR Protoc
Year:
2021
Journal volume:
2
Journal issue:
4
Fulltext / DOI:
doi:10.1016/j.xpro.2021.100877
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/34816125
TUM Institution:
Fachgebiet Neuroradiologie (Prof. Zimmer); Institut für Neurowissenschaften
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