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- Original title:
- Identification of context-specific mRNA translation regulators in human cells by functional genomics
- Translated title:
- Identifizierung kontextspezifischer mRNA-Translationsregulatoren in menschlichen Zellen durch funktionelle Genomanalyse
- Author:
- Rodschinka, Geraldine Y. V.
- Year:
- 2022
- Document type:
- Dissertation
- Faculty/School:
- TUM School of Natural Sciences
- Advisor:
- Nedialkova, Danny (Prof. Dr.)
- Referee:
- Nedialkova, Danny (Prof. Dr.); Murray, Peter (Prof., Ph.D.); Stingele, Julian (Prof. Dr.)
- Language:
- en
- Subject group:
- CHE Chemie
- TUM classification:
- CHE 800
- Abstract:
- Compromised function of the mRNA translation machinery often leads to tissue-specific diseases. To understand the underlying mechanisms, we queried the importance of its components in proliferating and maturated human cells with CRISPRi screens. The loss of translation-dependent quality control factors leads to phenotypes that are strongly context-dependent. Furthermore, we identified new endogenous human mRNAs that are substrates of co-translational quality control pathways.
- Translated abstract:
- Eine gestörte Funktion der mRNA-Translationsmaschinerie führt häufig zu gewebespezifischen Krankheiten. Um die zugrundeliegenden Mechanismen zu verstehen, haben wir ihrer Komponenten in proliferierenden und adulten menschlichen Zellen mithilfe CRISPRi-Screens untersucht. Der Verlust von Qualitätskontrollfaktoren führt zu Phänotypen, die stark kontextabhängig sind. Darüber hinaus haben wir neue endogene mRNAs identifiziert, die Substrate dieser co-translationalen Qualitätskontrolle sind.
- WWW:
- https://mediatum.ub.tum.de/?id=1686251
- Date of submission:
- 29.08.2022
- Oral examination:
- 02.11.2022
- File size:
- 9731598 bytes
- Pages:
- 172
- Urn (citeable URL):
- https://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:91-diss-20221102-1686251-1-6
- Last change:
- 02.11.2023
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