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- Original title:
- Structural studies on the pore-forming mitochondrial membrane proteins Bak and MPV17
- Translated title:
- Strukturelle Charakterisierung der Poren-bildenden mitochondrialen Membranproteine Bak und MPV17
- Author:
- Sperl, Laura Esther
- Year:
- 2021
- Document type:
- Dissertation
- Faculty/School:
- Fakultät für Chemie
- Advisor:
- Hagn, Franz (Prof. Dr.)
- Referee:
- Hagn, Franz (Prof. Dr.); Sattler, Michael (Prof. Dr.)
- Language:
- en
- Subject group:
- CHE Chemie
- Keywords:
- Bak, MPV17, membrane protein, nanodisc, NMR, mitochondria, apoptosis
- Translated keywords:
- Bak, MPV17, Membran Protein, Nanodisc, NMR, Mitochondrien, Apoptosis
- TUM classification:
- CHE 887
- Abstract:
- The disease-related mitochondrial membrane proteins Bak and MPV17 are studied in lipid bilayers by NMR and other biophysical methods. For pro-apoptotic Bak, both its pore-forming and its resting state, as well as its interaction with the anti-apoptotic player BclxL are characterized in nanodiscs and liposomes. For MPV17, NMR studies on the monomer are conducted in micelles, while the presumably pore-forming oligomer is studied in a lipid bilayer revealing an important role for cysteines.
- Translated abstract:
- Die mitochondrialen Membranproteine Bak und MPV17 werden u.a. mittels NMR in einer Membranumgebung studiert. Für pro-apoptotisches Bak wird sowohl der inaktive als auch der aktive Poren-bildende Zustand und die Interaktion mit dem anti-apoptotischen BclxL in Nanodiscs und Liposomen untersucht. Monomeres MPV17 wird mittels NMR in Mizellen und das vermutlich Poren-bildende Oligomer in einer Lipid-Doppenschicht untersucht. Dabei konnte den Cysteinen eine wichtige Rolle zugeordnet werden.
- WWW:
- https://mediatum.ub.tum.de/?id=1618020
- Date of submission:
- 09.08.2021
- Oral examination:
- 24.09.2021
- File size:
- 14495367 bytes
- Pages:
- 211
- Urn (citeable URL):
- https://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:91-diss-20210924-1618020-1-1
- Last change:
- 24.09.2022
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