Glycosyltransferases form oligo- and homopolysacchrides from sucrose. The regulation and functionality von glycosyltransferases in two strains of Lactobacillus reuteri, L. reuteri TMW1.106 and LTH5448, was examined. Fructosyltransferases were regulated by sucrose and by environmental conditions. Products formed by fructosyltransferases improved stress resistance towards antimicrobial substances and during freeze-drying. The presence of fructosyltransferases increased competitiveness of L. reuteri in the natural habitats sourdough and mice forestomach. The expression of the glucosyltransferase GtfA of L. reuteri TMW1.106 was not affected by sucrose or environmental conditions. Inactivation of GtfA had no impact on the performance of L. reuteri TMW1.106 neither in sourdough nor in the mice forestomach. Nevertheless, glucosyltransferases might play a role in other ecosystems.
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Glycosyltransferases form oligo- and homopolysacchrides from sucrose. The regulation and functionality von glycosyltransferases in two strains of Lactobacillus reuteri, L. reuteri TMW1.106 and LTH5448, was examined. Fructosyltransferases were regulated by sucrose and by environmental conditions. Products formed by fructosyltransferases improved stress resistance towards antimicrobial substances and during freeze-drying. The presence of fructosyltransferases increased competitiveness of L. reuter...
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