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Title:

Ex vivo immunocapture and functional characterization of cell-type-specific mitochondria using MitoTag mice.

Document type:
Article; Journal Article; Review; Research Support, Non-U.S. Gov't
Author(s):
de Mello, Natalia Prudente; Fecher, Caroline; Pastor, Adrian Marti; Perocchi, Fabiana; Misgeld, Thomas
Abstract:
Mitochondria are key bioenergetic organelles involved in many biosynthetic and signaling pathways. However, their differential contribution to specific functions of cells within complex tissues is difficult to dissect with current methods. The present protocol addresses this need by enabling the ex vivo immunocapture of cell-type-specific mitochondria directly from their tissue context through a MitoTag reporter mouse. While other available methods were developed for bulk mitochondria isolation...     »
Journal title abbreviation:
Nat Protoc
Year:
2023
Journal volume:
18
Journal issue:
7
Pages contribution:
2181-2220
Fulltext / DOI:
doi:10.1038/s41596-023-00831-w
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/37328604
Print-ISSN:
1754-2189
TUM Institution:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)
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