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Titel:

Ex vivo immunocapture and functional characterization of cell-type-specific mitochondria using MitoTag mice.

Dokumenttyp:
Article; Journal Article; Review; Research Support, Non-U.S. Gov't
Autor(en):
de Mello, Natalia Prudente; Fecher, Caroline; Pastor, Adrian Marti; Perocchi, Fabiana; Misgeld, Thomas
Abstract:
Mitochondria are key bioenergetic organelles involved in many biosynthetic and signaling pathways. However, their differential contribution to specific functions of cells within complex tissues is difficult to dissect with current methods. The present protocol addresses this need by enabling the ex vivo immunocapture of cell-type-specific mitochondria directly from their tissue context through a MitoTag reporter mouse. While other available methods were developed for bulk mitochondria isolation...     »
Zeitschriftentitel:
Nat Protoc
Jahr:
2023
Band / Volume:
18
Heft / Issue:
7
Seitenangaben Beitrag:
2181-2220
Volltext / DOI:
doi:10.1038/s41596-023-00831-w
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/37328604
Print-ISSN:
1754-2189
TUM Einrichtung:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)
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