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Titel:

Genome-scale pan-cancer interrogation of lncRNA dependencies using CasRx.

Dokumenttyp:
Journal Article
Autor(en):
Montero, Juan J; Trozzo, Riccardo; Sugden, Maya; Öllinger, Rupert; Belka, Alexander; Zhigalova, Ekaterina; Waetzig, Paul; Engleitner, Thomas; Schmidt-Supprian, Marc; Saur, Dieter; Rad, Roland
Abstract:
Although long noncoding RNAs (lncRNAs) dominate the transcriptome, their functions are largely unexplored. The extensive overlap of lncRNAs with coding and regulatory sequences restricts their systematic interrogation by DNA-directed perturbation. Here we developed genome-scale lncRNA transcriptome screening using Cas13d/CasRx. We show that RNA targeting overcomes limitations inherent to other screening methods, thereby considerably expanding the explorable space of the lncRNAome. By evolving th...     »
Zeitschriftentitel:
Nat Methods
Jahr:
2024
Band / Volume:
21
Heft / Issue:
4
Seitenangaben Beitrag:
584-596
Volltext / DOI:
doi:10.1038/s41592-024-02190-0
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/38409225
Print-ISSN:
1548-7091
TUM Einrichtung:
Professur für Molekulare Onkologie und Funktionelle Genomik (Prof. Rad)
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