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Title:

CRISPR somatic genome engineering and cancer modeling in the mouse pancreas and liver.

Document type:
Journal Article; Review; Research Support, Non-U.S. Gov't
Author(s):
Kaltenbacher, Thorsten; Löprich, Jessica; Maresch, Roman; Weber, Julia; Müller, Sebastian; Oellinger, Rupert; Groß, Nina; Griger, Joscha; de Andrade Krätzig, Niklas; Avramopoulos, Petros; Ramanujam, Deepak; Brummer, Sabine; Widholz, Sebastian A; Bärthel, Stefanie; Falcomatà, Chiara; Pfaus, Anja; Alnatsha, Ahmed; Mayerle, Julia; Schmidt-Supprian, Marc; Reichert, Maximilian; Schneider, Günter; Ehmer, Ursula; Braun, Christian J; Saur, Dieter; Engelhardt, Stefan; Rad, Roland
Abstract:
Genetically engineered mouse models (GEMMs) transformed the study of organismal disease phenotypes but are limited by their lengthy generation in embryonic stem cells. Here, we describe methods for rapid and scalable genome engineering in somatic cells of the liver and pancreas through delivery of CRISPR components into living mice. We introduce the spectrum of genetic tools, delineate viral and nonviral CRISPR delivery strategies and describe a series of applications, ranging from gene editing...     »
Journal title abbreviation:
Nat Protoc
Year:
2022
Journal volume:
17
Journal issue:
4
Pages contribution:
1142-1188
Fulltext / DOI:
doi:10.1038/s41596-021-00677-0
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/35288718
Print-ISSN:
1754-2189
TUM Institution:
611; Experimentelle Hämatologie (Prof. Schmidt-Supprian); Institut für Pharmakologie und Toxikologie; Klinik und Poliklinik für Innere Medizin II, Gastroenterologie; Lehrstuhl für Translationale Tumorforschung (DKTK) (Prof. Saur); Professur für Molekulare Onkologie und Funktionelle Genomik (Prof. Rad)
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