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Title:

Live-Cell Imaging of RNA Transport in Axons of Cultured Primary Neurons.

Document type:
Journal Article
Author(s):
Hees, J Tabitha; Harbauer, Angelika B
Abstract:
The use of fluorescent proteins has revolutionized the study of protein localization and transport. However, the visualization of other molecules and specifically RNA during live-cell imaging remains challenging. In this chapter, we provide guidance to the available methods, their advantages and drawbacks as well as provide a detailed protocol for the detection of RNA transport using the MS2/PP7-split-Venus system for background-free RNA imaging.
Journal title abbreviation:
Methods Mol Biol
Year:
2022
Journal volume:
2431
Pages contribution:
225-237
Fulltext / DOI:
doi:10.1007/978-1-0716-1990-2_11
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/35412279
Print-ISSN:
1064-3745
TUM Institution:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)
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