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Titel:

Isolation and Electron Microscopic Analysis of Liver Cancer Cell Mitochondria.

Dokumenttyp:
Journal Article
Autor(en):
Kabiri, Yaschar; Eberhagen, Carola; Schmitt, Sabine; Knolle, Percy A; Zischka, Hans
Abstract:
Isolation of mitochondria is a crucial method for examining molecular details of this organelle's manifold functions. Historically, mitochondrial isolations required large amounts of sample material which impeded their isolation from cultured cells. We have therefore developed a method allowing for controlled and reproducible isolation of intact and functional mitochondria from diverse cell types in culture. Here we provide a methodological update of this approach together with a protocol for th...     »
Zeitschriftentitel:
Methods Mol Biol
Jahr:
2021
Band / Volume:
2277
Seitenangaben Beitrag:
277-287
Volltext / DOI:
doi:10.1007/978-1-0716-1270-5_17
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/34080157
Print-ISSN:
1064-3745
TUM Einrichtung:
Institut für Toxikologie und Umwelthygiene; Roman Herzog Comprehensive Cancer Center
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