Meat allergy associated with galactosyl-?-(1,3)-galactose (?-Gal)-Closing diagnostic gaps by anti-?-Gal IgE immune profiling.

Jappe, U; Minge, S; Kreft, B; Ludwig, A; Przybilla, B; Walker, A; Varga, R; Seidel, P; Biedermann, T; Anemüller, W; Kromminga, A; Ruëff, F; Merk, H; Wagner, N; Treudler, R; Worm, M; Waldmann, I; Saloga, J; Becker, W M; Goldmann, T; Platts-Mills, T A; Homann, A

doi:10.1111/all.13238

Benutzer: Gast

journal article

Dokumenttyp:: Journal Article; Article
Autor(en):: Jappe, U; Minge, S; Kreft, B; Ludwig, A; Przybilla, B; Walker, A; Varga, R; Seidel, P; Biedermann, T; Anemüller, W; Kromminga, A; Ruëff, F; Merk, H; Wagner, N; Treudler, R; Worm, M; Waldmann, I; Saloga, J; Becker, W M; Goldmann, T; Platts-Mills, T A; Homann, A
Titel:: Meat allergy associated with galactosyl-?-(1,3)-galactose (?-Gal)-Closing diagnostic gaps by anti-?-Gal IgE immune profiling.
Abstract:: Glycoproteins and glycolipids of some mammalian species contain the disaccharide galactosyl-?-(1,3)-galactose (?-Gal). It is known that ?-Gal is immunogenic in humans and causes glycan-specific IgG and also IgE responses with clinical relevance. ?-Gal is part of the IgE-reactive monoclonal therapeutic antibody cetuximab (CTX) and is associated with delayed anaphylaxis to red meat. In this study, different ?-Gal-containing analytes are examined in singleplex and multiplex assays to resolve individual sensitization patterns with IgE against ?-Gal.Three serum groups, ?-Gal-associated meat allergy (MA) patients, idiopathic anaphylaxis (IA) patients with suspected MA, and non-meat-allergic healthy control individuals (HC), were analyzed via singleplex allergy diagnostics and a newly established immunoblot diagnostic system. The new dot blot detection system resolved individual IgE sensitization profiles for ?-Gal-containing analytes CTX, bovine thyroglobulin (Bos d TG), and human serum albumin (HSA)-conjugated ?-Gal.Singleplex allergy diagnostics using the ?-Gal analytes CTX and Bos d TG confirms the history of MA patients in 91% and 88% of the cases, respectively. A novel dot blot-based assay system for the detection of IgE against ?-Gal reveals individual IgE sensitization profiles for ?-Gal-containing analytes. An ?-Gal-associated IgE cross-reactivity profile (IgE against CTX, Bos d TG, and HSA-?-Gal) was identified, which is associated with MA.Detection of individual sensitization patterns with different ?-Gal-containing analytes provides the basis for an individual allergy diagnosis for ?-Gal-sensitized patients. Higher amounts of ?-Gal in pork and beef innards compared to muscle meat as indicated by a higher staining intensity are a plausible explanation for the difference in allergic symptom severity. «
Glycoproteins and glycolipids of some mammalian species contain the disaccharide galactosyl-?-(1,3)-galactose (?-Gal). It is known that ?-Gal is immunogenic in humans and causes glycan-specific IgG and also IgE responses with clinical relevance. ?-Gal is part of the IgE-reactive monoclonal therapeutic antibody cetuximab (CTX) and is associated with delayed anaphylaxis to red meat. In this study, different ?-Gal-containing analytes are examined in singleplex and multiplex assays to resolve indivi... »
Zeitschriftentitel:: Allergy
Jahr:: 2018
Band / Volume:: 73
Heft / Issue:: 1
Seitenangaben Beitrag:: 93-105
Volltext / DOI:: doi:10.1111/all.13238
PubMed:: http://view.ncbi.nlm.nih.gov/pubmed/28670695
Print-ISSN:: 0105-4538
TUM Einrichtung:: Klinik und Poliklinik für Dermatologie und Allergologie
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mediaTUM Gesamtbestand Einrichtungen Schools TUM School of Medicine and Health Departments Clinical Medicine Klinik und Poliklinik für Dermatologie und Allergologie (Prof. Biedermann)2018