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- Title:
Assessment of urinary 3-indoxyl sulfate as a marker for gut microbiota diversity and abundance of Clostridiales.
- Document type:
- Journal Article
- Author(s):
- Farowski, Fedja; Els, Gregor; Tsakmaklis, Anastasia; Higgins, Paul G; Kahlert, Christian R; Stein-Thoeringer, Christoph K; Bobardt, Johanna S; Dettmer-Wilde, Katja; Oefner, Peter J; Vehreschild, Jörg Janne; Vehreschild, Maria J G T
- Abstract:
- After allogeneic hematopoietic stem cell transplantation (allo-HCT), urinary levels of 3-indoxyl sulfate (3-IS) correlate with the relative abundance of bacteria from the class Clostridia (RAC), and antibiotic treatment is considered the major determinant of this outcome. A high RAC has been associated with favorable outcome after allo-HCT and protection from Clostridium difficile infection (CDI). We assessed correlations between alpha diversity, RAC and urinary 3-IS levels in a non-allo-HCT clinical cohort of antibiotic treated patients to further explore 3-IS as a biomarker of reduced diversity and predisposition to CDI.Fecal and urinary specimens were analyzed from 40 non-allo-HCT hospitalized patients before and 9 ± 2 days after initiation of intravenous antibiotic treatment. Fecal microbiota were analyzed by 16s RNA sequencing and urinary 3-IS was analyzed by liquid chromatography-tandem mass spectrometry. Receiver operating characteristic (ROC) analysis was performed to assess the predictive value of 3-IS.At a RAC cutoff of <30%, the binary logarithm of 3-IS (medium 3-IS: <=2.5; high 3-IS: >2.5) was predictive with an accuracy of 82% (negative predictive value: 87%, positive predictive value 67%). Accuracy was improved by combing antibiotic history with 3-IS levels (accuracy 89%, npv 88%, ppv 92%).In conjunction with patient antibiotic history, 3-IS is a candidate marker to predict RAC.
- Journal title abbreviation:
- Gut Microbes
- Year:
- 2019
- Journal volume:
- 10
- Journal issue:
- 2
- Pages contribution:
- 133-141
- Fulltext / DOI:
- doi:10.1080/19490976.2018.1502536
- Pubmed ID:
- http://view.ncbi.nlm.nih.gov/pubmed/30118620
- Print-ISSN:
- 1949-0976
- TUM Institution:
- II. Medizinische Klinik und Poliklinik (Gastroenterologie)
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