Expanding the DNA origami construction space demands the use of “scaffold” DNA single strands with user-defined lengths and sequences. The biotechnological methods developed in this thesis facilitate the production of synthetic single-stranded DNA with designed sequence properties. By implementing these sequences as custom scaffolds, one can increase assembly precision of DNA objects and gain variability in object size and nucleobase composition. The inclusion of sequence segments with appropriate attributes enables the creation of DNA objects with defined functionality.
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Expanding the DNA origami construction space demands the use of “scaffold” DNA single strands with user-defined lengths and sequences. The biotechnological methods developed in this thesis facilitate the production of synthetic single-stranded DNA with designed sequence properties. By implementing these sequences as custom scaffolds, one can increase assembly precision of DNA objects and gain variability in object size and nucleobase composition. The inclusion of sequence segments with appropria...
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