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Document type:
Zeitschriftenaufsatz
Author(s):
Kaufmann, Christine M.; Graßmann, Johanna; Treutter, Dieter; Letzel, Thomas
Title:
Utilization of real-time electrospray ionization mass spectrometry to gain further insight into the course of nucleotide degradation by intestinal alkaline phosphatase
Abstract:
RATIONALE Related with its ability to degrade nucleotides, intestinal alkaline phosphatase (iAP) is an important participant in intestinal pH regulation and inflammatory processes. However, its activity has been investigated mainly by using artificial non-nucleotide substrates to enable the utilization of conventional colorimetric methods. To capture the degradation of the physiological nucleotide substrate of the enzyme along with arising intermediates and the final product, the enzymatic assa...     »
Keywords:
Alkaline Phosphatase/analysis/metabolism; Animals; Cattle; Colorimetry; Enzyme Assays/methods; Intestinal Mucosa/enzymology; Kinetics; Spectrometry, Mass, Electrospray Ionization/methods
Journal title:
Rapid communications in mass spectrometry : RCM
Year:
2014
Journal volume:
28
Journal issue:
8
Pages contribution:
869--878
Fulltext / DOI:
doi:10.1002/rcm.6855
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